rabbit polyclonal anti-human desmin  (R&D Systems)

Journal: Purinergic Signalling

Article Title: The ecto-enzymes CD73 and adenosine deaminase modulate 5′-AMP-derived adenosine in myofibroblasts of the rat small intestine

doi: 10.1007/s11302-018-9623-6

Figure Lengend Snippet: Immunohistochemical characterization of rat IMFs. a Representative confocal microphotographs showing the distribution of vimentin (green) and desmin (red) in rat IMFs. b Representative confocal microphotographs showing the distribution of α-SMA (green) and desmin (red) in rat IMFs. Isotype fluorescent images were obtained by labeling with Alexa Fluor 488 and Alexa Fluor 555 conjugated secondary antibodies in presence of nonimmune rabbit or mouse antiserum instead of the primary antibodies. Cell nuclei were stained with DAPI (blue). n = 6. Scale bars = 25 μm

Article Snippet: Fixed cells were rinsed with PBS, permeabilized with 0.1% Triton X-100, and incubated with the following primary antibodies: rabbit polyclonal anti-human desmin (1:100, Thermo Fisher Scientific, Milan, Italy), mouse monoclonal anti-human vimentin (1:100, clone V9, Dako, Italy), mouse monoclonal α-SMA FITC-conjugated (1:200; clone 1A4, Sigma Aldrich, Milan, Italy), mouse anti-rat CD73 (1:500; BD Biosciences Pharmigen, Belgium), and biotin-labeled rabbit anti-bovine ADA (1:100; Alpha Diagnostic International Inc., USA), diluted in PBS containing 0.1% BSA and 10% normal goat serum for 1 h at room temperature [ 10 , 23 , 24 ].

Techniques: Immunohistochemical staining, Labeling, Staining